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primary human coronary artery vsmcs  (Lonza)


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    Lonza primary human coronary artery vsmcs
    Primary Human Coronary Artery Vsmcs, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human coronary artery vsmcs/product/Lonza
    Average 90 stars, based on 1 article reviews
    primary human coronary artery vsmcs - by Bioz Stars, 2026-03
    90/100 stars

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    F actin is reduced in diabetic CRMs: (A) F actin content in control and diabetic mouse coronary <t>VSMCs</t> obtained via staining with phalloidin. (B) G actin levels in control and <t>T2DM</t> mouse coronary VSMCs attained through staining with DNase. (C) F/G actin ratio of mouse normal and diabetic coronary VSMCs. (D) Representative images from Db/db (top) and db/db (bottom). Data are mean ± SEM. **p ≤ 0.005. n = 3 per group.
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    F actin is reduced in diabetic CRMs: (A) F actin content in control and diabetic mouse coronary VSMCs obtained via staining with phalloidin. (B) G actin levels in control and T2DM mouse coronary VSMCs attained through staining with DNase. (C) F/G actin ratio of mouse normal and diabetic coronary VSMCs. (D) Representative images from Db/db (top) and db/db (bottom). Data are mean ± SEM. **p ≤ 0.005. n = 3 per group.

    Journal: Frontiers in Physiology

    Article Title: Coronary cytoskeletal modulation of coronary blood flow in the presence and absence of type 2 diabetes: the role of cofilin

    doi: 10.3389/fphys.2025.1561867

    Figure Lengend Snippet: F actin is reduced in diabetic CRMs: (A) F actin content in control and diabetic mouse coronary VSMCs obtained via staining with phalloidin. (B) G actin levels in control and T2DM mouse coronary VSMCs attained through staining with DNase. (C) F/G actin ratio of mouse normal and diabetic coronary VSMCs. (D) Representative images from Db/db (top) and db/db (bottom). Data are mean ± SEM. **p ≤ 0.005. n = 3 per group.

    Article Snippet: Deidentified normal and T2DM human primary coronary VSMCs were obtained from both Lonza (Morristown, NJ) and ATCC (Manassas, VA).

    Techniques: Control, Staining

    Cofilin is increased in T2DM coronary microvessels: (A) Proteomics analysis of cofilin spectral hits in the normal CRMs db/db CRMs. (B) Cofilin protein expression obtained via western blot analysis in the coronary VSMCs of normal and diabetic human patients. Data are mean ± SEM. p-values are presented on the graph and *p < 0.05 and ****p ≤ 0.0001. n = 3-5 per group.

    Journal: Frontiers in Physiology

    Article Title: Coronary cytoskeletal modulation of coronary blood flow in the presence and absence of type 2 diabetes: the role of cofilin

    doi: 10.3389/fphys.2025.1561867

    Figure Lengend Snippet: Cofilin is increased in T2DM coronary microvessels: (A) Proteomics analysis of cofilin spectral hits in the normal CRMs db/db CRMs. (B) Cofilin protein expression obtained via western blot analysis in the coronary VSMCs of normal and diabetic human patients. Data are mean ± SEM. p-values are presented on the graph and *p < 0.05 and ****p ≤ 0.0001. n = 3-5 per group.

    Article Snippet: Deidentified normal and T2DM human primary coronary VSMCs were obtained from both Lonza (Morristown, NJ) and ATCC (Manassas, VA).

    Techniques: Expressing, Western Blot

    Cofilin knockdown causes altered F/G actin ratio and a significant increase in stiffness in human coronary VSMCs: (A) F/G actin ratio of normal and diabetic combined human coronary VSMCs treated with cofilin siRNA obtained via staining F actin (phalloidin) and G actin (DNase). (B) F/G actin ratio of cofilin siRNA treated normal coronary VSMCs. (C) F/G actin ratio of human diabetic coronary VSMCs treated with cofilin siRNA. (D) Elastic modulus (E inc ) of normal and diabetic combined human coronary VSMCs treated with cofilin siRNA acquired via atomic force microscopy. (E) Elastic modulus of cofilin siRNA treated human normal coronary VSMCs. (F) Elastic modulus of human diabetic coronary VSMCs treated with cofilin siRNA. Data are mean ± SEM. *p < 0.05. n = 4-8 per group.

    Journal: Frontiers in Physiology

    Article Title: Coronary cytoskeletal modulation of coronary blood flow in the presence and absence of type 2 diabetes: the role of cofilin

    doi: 10.3389/fphys.2025.1561867

    Figure Lengend Snippet: Cofilin knockdown causes altered F/G actin ratio and a significant increase in stiffness in human coronary VSMCs: (A) F/G actin ratio of normal and diabetic combined human coronary VSMCs treated with cofilin siRNA obtained via staining F actin (phalloidin) and G actin (DNase). (B) F/G actin ratio of cofilin siRNA treated normal coronary VSMCs. (C) F/G actin ratio of human diabetic coronary VSMCs treated with cofilin siRNA. (D) Elastic modulus (E inc ) of normal and diabetic combined human coronary VSMCs treated with cofilin siRNA acquired via atomic force microscopy. (E) Elastic modulus of cofilin siRNA treated human normal coronary VSMCs. (F) Elastic modulus of human diabetic coronary VSMCs treated with cofilin siRNA. Data are mean ± SEM. *p < 0.05. n = 4-8 per group.

    Article Snippet: Deidentified normal and T2DM human primary coronary VSMCs were obtained from both Lonza (Morristown, NJ) and ATCC (Manassas, VA).

    Techniques: Knockdown, Staining, Microscopy

    Cofilin siRNA disrupts the F/G actin ratio and increases stiffness in mouse coronary VMSCs: (A) F/G actin ratio of mouse normal and diabetic combined coronary VSMCs treated with cofilin siRNA obtained via staining F actin (phalloidin) and G actin (DNase). (B) Normal control mouse coronary VSMC F/G actin ratio after cofilin siRNA treatment. (C) F/G actin ratio of mouse diabetic coronary VSMCs treated with cofilin siRNA. (D) Normal and diabetic combined mouse coronary VSMCs treated with cofilin siRNA elastic modulus (E inc ) data obtained using atomic force microscopy. (E) Elastic modulus of cofilin siRNA treated normal control mouse coronary VSMCs. (F) Elastic modulus of mouse diabetic coronary VSMCs treated with cofilin siRNA. Data are mean ± SEM. **p < 0.005, ***p < 0.0005, and ****p < 0.0001 vs. respective scramble control. n = 2–10 per group.

    Journal: Frontiers in Physiology

    Article Title: Coronary cytoskeletal modulation of coronary blood flow in the presence and absence of type 2 diabetes: the role of cofilin

    doi: 10.3389/fphys.2025.1561867

    Figure Lengend Snippet: Cofilin siRNA disrupts the F/G actin ratio and increases stiffness in mouse coronary VMSCs: (A) F/G actin ratio of mouse normal and diabetic combined coronary VSMCs treated with cofilin siRNA obtained via staining F actin (phalloidin) and G actin (DNase). (B) Normal control mouse coronary VSMC F/G actin ratio after cofilin siRNA treatment. (C) F/G actin ratio of mouse diabetic coronary VSMCs treated with cofilin siRNA. (D) Normal and diabetic combined mouse coronary VSMCs treated with cofilin siRNA elastic modulus (E inc ) data obtained using atomic force microscopy. (E) Elastic modulus of cofilin siRNA treated normal control mouse coronary VSMCs. (F) Elastic modulus of mouse diabetic coronary VSMCs treated with cofilin siRNA. Data are mean ± SEM. **p < 0.005, ***p < 0.0005, and ****p < 0.0001 vs. respective scramble control. n = 2–10 per group.

    Article Snippet: Deidentified normal and T2DM human primary coronary VSMCs were obtained from both Lonza (Morristown, NJ) and ATCC (Manassas, VA).

    Techniques: Staining, Control, Microscopy

    Latrunculin B treatment of coronary VSMCs significantly reduces cellular stiffness: (A) Elastic modulus acquired via atomic force microscopy of LatB treated normal and diabetic human coronary VSMCs. (B) LatB treated mouse normal and diabetic coronary VSMC elastic modulus. Data are mean ± SEM. *p < 0.05, **p < 0.005, ***p < 0.0005, and ****p < 0.0001. n = 2-5 per group.

    Journal: Frontiers in Physiology

    Article Title: Coronary cytoskeletal modulation of coronary blood flow in the presence and absence of type 2 diabetes: the role of cofilin

    doi: 10.3389/fphys.2025.1561867

    Figure Lengend Snippet: Latrunculin B treatment of coronary VSMCs significantly reduces cellular stiffness: (A) Elastic modulus acquired via atomic force microscopy of LatB treated normal and diabetic human coronary VSMCs. (B) LatB treated mouse normal and diabetic coronary VSMC elastic modulus. Data are mean ± SEM. *p < 0.05, **p < 0.005, ***p < 0.0005, and ****p < 0.0001. n = 2-5 per group.

    Article Snippet: Deidentified normal and T2DM human primary coronary VSMCs were obtained from both Lonza (Morristown, NJ) and ATCC (Manassas, VA).

    Techniques: Microscopy

    Latrunculin B increases CBF: (A) Baseline CBF obtained from normal and T2DM hearts ex vivo on the Langendorff perfusion system. (B) Peak responses to ML-7 and ML-7 + LatB of ex vivo heart on Langendorff perfusion system. Data are mean ± SEM. *p < 0.05, **p < 0.005. n = 11 per group.

    Journal: Frontiers in Physiology

    Article Title: Coronary cytoskeletal modulation of coronary blood flow in the presence and absence of type 2 diabetes: the role of cofilin

    doi: 10.3389/fphys.2025.1561867

    Figure Lengend Snippet: Latrunculin B increases CBF: (A) Baseline CBF obtained from normal and T2DM hearts ex vivo on the Langendorff perfusion system. (B) Peak responses to ML-7 and ML-7 + LatB of ex vivo heart on Langendorff perfusion system. Data are mean ± SEM. *p < 0.05, **p < 0.005. n = 11 per group.

    Article Snippet: Deidentified normal and T2DM human primary coronary VSMCs were obtained from both Lonza (Morristown, NJ) and ATCC (Manassas, VA).

    Techniques: Ex Vivo

    Role of miRNA in various metabolic pathways

    Journal: World Journal of Clinical Cases

    Article Title: Emerging roles of microRNAs as diagnostics and potential therapeutic interest in type 2 diabetes mellitus

    doi: 10.12998/wjcc.v12.i3.525

    Figure Lengend Snippet: Role of miRNA in various metabolic pathways

    Article Snippet: 9 , miR-21-3p , PKC , Human aortic vsmcs (C-12511, promocell, and human vets , Diabetic atherosclerosis , Down-regulated , [ ] .

    Techniques: